PPB

 

PPB
Information for Physicians

 

 

Instructions for Pathologists

Anticoagulated Whole Blood for Peripheral Leukocyte Collection

These specimens are used as a source of germ line (non-tumor) DNA for comparative genomic analysis.  In this case, the timing of specimen collection relative to tumor resection is not critical.  It is best to collect the specimen at a time when the participant is not neutropenic.
The clinical staff should collect three, 10cc tubes of peripheral blood by standard venous phlebotomy (For small children, two tubes are sufficient.)  Blood may be collected in the three ACD (yellow top) tubes provided in the collection kit or three EDTA (purple top) tubes that are pre-labeled with the patient's name. After collection, the tubes should be mixed several times and labeled with the collection date and time.  Specimens should be shipped according to the instructions provided in the kit.

Snap Frozen Tumor and Normal Tissue

Preparation of tissue for preservation at -80°C

  1. As soon as possible after tumor resection and after the proper gross examination select areas of viable tumor and viable normal tissue not critical for diagnosis to snap freeze (several fragments of tissue if possible). For cases of Type I (cystic) PPB, take several samples of cyst wall. For cases of Type II (cystic and solid) PPB, take samples of cyst wall and samples of solid, viable tumor.
  2. Cut samples into small chunks not larger than 1 cm3.  Place in appropriate freezing bag. Please label bag/cassette with patient’s name and/or surgical identification number and “T” or “N” for Tumor or Normal.  If using a tissue cassette, wrap cassette in foil.
  3. Drop bag/foil-wrapped cassette into liquid nitrogen or refrigerated cryobath for at least 5 minutes.
  4. Place bag/cassette into one or more of the ziplock bags provided that is legibly labeled with the following.
    1. Patient’s full name and “PPB Study”
    2. Tissue site
    3. Tumor or Non-malignant tissue
  5. Place bag into -70°C freezer or in dry ice until ready for shipping. Take extreme care not to let the tissue specimen thaw.

Note: We also encourage submission of samples of other tumors or diseased tissue in addition to PPB (e.g. cystic nephroma, thyroid, etc…).


Questions?  Call D. Ashley Hill, M.D.  (202) 476-2815 or (202) 476-2051

 

 
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Washington University Barnes Jewish Hospital Siteman Cancer Center
Children's Hospital

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